Opioid Receptors: Methods and Protocols serves as a comprehensive guide to both key new techniques and established methods for the investigation of genetics, structural biology, transcription, and post-transcriptional events of opioid receptors. Other methods cover the cellular detection and trafficking of opioid receptors in vitro and in vivo. Procedures aimed to investigate signaling pathways modulated by opioid receptors and model systems to study opioid receptor-mediated functions are also included. Finally, methods to assay behavioral effects mediated by opioid receptors are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Wide-ranging and authoritative, Opioid Receptors: Methods and Protocols will help both experienced and new entrants in this field to carry out their experiments successfully and with new inspiration.
(Chapter 1 ). The recent publication of crystal structures of all the three opioid receptors has been instrumental to the development of computational protocols, designed to estimate thermodynamic and kinetic parameters describing the receptor binding of small molecule ligands and the formation of supramolecular complexes.
(Chapter 2 ). Furthermore, techniques for the epigenetic and posttranscriptional analysis of opioid receptor genes are presented.
(Chapters 3 and 4 ). Finally, a protocol is dedicated to the use of DNA microarrays and next-generation sequencing methodologies to obtain a transcriptional profi le of genes influenced by activation of opioid receptors.